Updates to "Pfizer Didn't Know What the Shots Do in the Body"
Revelations from Patel, et al.
As reported by Jessica Rose, Pfizer self-published the very same simulated blots involved in “Blot-gate” on January 12:
The study:
There’s nothing truly “new;” this paper publishes the same experiments with the same images mailed to regulators so they could perfunctorily pretend to regulate, but a bit more detail on methods.
Accordingly, I have made small updates to Sunday’s post to include the minor revelations disclosed in Patel, et al. The following review was added in the footnotes:
Patel, HK. et al. “Characterization of BNT162b2 mRNA to Evaluate Risk of Off-Target Antigen Translation.” Journal of Pharmaceutical Sciences.
Supports my interpretation of the regulators being pre-aware of the processing step. Patel, et al. casually report that for the experiments besides the cell-free degradation assay, “mRNA sample results are reported as ProteinSimple Wes assay run lane images.” Regulators probably knew this.
Contra my original text, ProteinSimple does constitute a tool to convert real blots directly to touched-up, simulated blots. i.e., the tool that I reported “should not exist,” exists (it is always helpful when brand names are specified). I have edited the text of this post with the original version preserved in strikethrough.
Reveals that Pfizer has either changed its mind or never was in agreement with the theory (prevailing in the reports submitted to regulators) that most partial RNAs were the product of degraded full RNAs. Patel, et al. now opine that most partial RNAs are incomplete transcriptions. This conclusion is portrayed as stemming from the experiments in question, as if to imply that no further work has been done to investigate manufacturing quality besides these few experiments on pre-EUA batches:
The data demonstrate that BNT162b2 fragments originate predominantly from premature transcriptional stops during manufacturing and do not have the capacity for protein translation, as evidenced by orthogonal protein expression systems.
Today
I am finally going to make a post about the mythical thing called the “worry window” (mythical at least before the bivalent booster, which is being delivered when the previously-boosted are the virtually only ones being infected anyway).
So get those unsubscribe-fingers ready.
Brian, you've written before about feeling your work doesn't make much of a difference and your low popularity compared to more sensationalist narratives. Obviously that's because of your pesky cold water pouring - but some people appreciate it. I have enough scientific training to know the importance of the 'ugly fact' so please keep those irritating observations coming!
To the people who may be outraged at the incompetence of the regulators, and may enter in a depressing pattern of thinking involving how should they be prosecuted, I say this: What incentive does any Government in the World have in prosecuting itself?
There.
You cannot go lower than that.
Dear Depression, I win today!
People, I have saved you a few cycles of misery in your brains. Go out and play.