Feb 27, 2022·edited Feb 27, 2022Liked by Brian Mowrey
The cmRNA vaccines are coded with Pseudouridine, an isomer of Uridine. This means that the spike proteins will be isomeric as well (as will the antibodies). The result of this is that the situation is much worse than that described in the paper: the heat shock proteins.
Sars-Cov-2 and M. bovis include identical genome sequences which include heat shock proteins. During a thermal shock, these proteins will be activated (as will the binding isomeric antibodies), with huge consequences on the DNA transcription phenomenon.
“The response to thermal stress in yeast is one of the most dynamic examples of transcriptional control known. Within 1 to 5 min of temperature upshift (30°C to 39°C), dramatic changes in protein-DNA interactions take place within HSP gene promoters, and these are accompanied by equally dramatic increases in transcription.”
“Lipid mediated gene transfer (lipofection) has been widely used to transfer genes into various cell types (1–4). Lipofection works very well in many cell lines, resulting in high transient transfection efficiencies (our observations). However, the rate of DNA integration into the genome following lipid-mediated transfection is relatively low (5) as compared to other methods, such as retroviral systems. This inefficient integration has been thought to be a major disadvantage of plasmid vectors and has limited their use in gene therapy trials.
We have attempted to overcome this hurdle by achieving higher rates of stable integrants in lipid-mediated transfections through treating the transfected cells with a mild heat shock.”
“Another factor commonly overlooked is the fact that bacteria produce reverse transcriptase via retrons, suggesting possible viral-bacterial interaction. Indeed, LPS is known to strongly impact the process:
“When we stimulated spleen cells with lipopolysaccharide (LPS), L1 mRNA levels apparently increased about 4-fold in the presence of AID and about 17-fold in its absence””
There is also this: “the possibility of telomerase HTERT, which is a reverse transcriptase enzyme, having any potential to insert the mRNA spike protein sequence into telomeric DNA.”
From the wmcresearch website:
"SARS-CoV-2 MAY OR MAY NOT WRITE ITSELF INTO YOUR DNA, BUT IT IS ALMOST CERTAINLY CUTTING/PASTING YOUR GENES, WRITING THEM BACK “WRONG" INTO YOUR DNA. LINE-1 ELEMENTS MUST BE TESTED IN ALL THOSE WITH COVID.
Retrotransposons (also called Class I transposable elements or transposons via RNA intermediates) are a type of genetic component that copy and paste themselves into different genomic locations (transposon) by converting RNA back into DNA through the process reverse transcription using an RNA transposition Retrotransposons can be further subdivided into two subclasses: those with Long-Terminal Repeats (LTR) and those without (non-LTR). LTR elements, also known as endogenous retroviruses (ERVs), comprise ~8 % of the human genome. LTR ) elements are thought to be inactive in the human lineage. That is, until SARS-CoV-2 activates them. This is why the HERV-K protein has never before been seen circulating in the body."
I found the wmcresearch site but am not sure which article corresponds to the quotes particularly for the HERV-K.
Viable TEs activate during demethylation, at which point they regulate cell differentiation during embryogenesis, or in immune and nerve cells where they seem to regulate memory. So you don't really want them active in a random stomach cell, the injections (and maybe most infections) could certainly prompt this directly or indirectly (via cell stress) and since the injections don't automatically destroy the cell, it's an implicit cancer risk. Cells should not be metabolically hijacked, in my view there will probably never be a non-carcinogenic mRNA tech.
The injections can NEVER be mRNA, but only cmRNA. Had the vaccines been coded with Uracil, ALL of the vaccinees would have found themselves in less than 24 hours in the ICUs with severe cases of Covid-19, since then the lethal abs (REGN10987 and B38) would have been activated in large quantities.
Is Sars-Cov-2 mycobacterium or a virus? If it is not a virus, then the fact that bacteria produce reverse transcriptase via retrons (especially M. tuberculosis) should worry the researchers much more than the paper published a few days ago.
wmcresearch
Category: Genetics
Subsections:
Covid-19 and Retrotransposons
The Emergence of the HERV-W ENV Protein in Covid-19
Thanks for the update to that post. The type of cells that a researcher uses should always be scrutinized for their ability to either mimic what could happen in human models or if they are representative of the process or intended goals overall. The cancer cells may have been used for their shorter replication and because LNPs may aggregate within the liver but the dynamics are very different when you add things to a petri dish rather than inject it and see where it carries and what the entire organism does with the compound.
Regardless, the most important thing is that someone is willing to examine criticisms and evaluate them accordingly. Honestly, someone may come by and say that my correction itself is wrong, although I can see why there may be plenty of confusion. The post wording wasn't very direct in what they meant.
Like I said the study is interesting but I think we will need more evidence to determine the dangers or harms of such a finding, if it is reproducible in human models.
Hi Brian. Do you want to help me with genetic analysis for an article I want to write? I can advertise your substack in that article and give y ou proper credit, of course.
one must not forget that synthesis of the spike protein is also occurring at the same time.If presented to the adjacent cells or even in the medium ,several pathways may be activated ,be it via TLRs 2 or 4 or others such as CD 209/ L-SIGN.Another publication ( not yet peer reviewed) presented evidence for activation of HERV-W in some volunteer lymphocytes upon spike exposure.There could be also ER stress.
It's a bit of a chicken vs egg phenomenon. Would the cells be forced to produce spike protein in the short run, and then have the mRNA be reverse transcribed? Also, if the cells are made to express spike proteins on their outer membranes we may also assume that in animal or human models the immune system would be able to recognize the antigen and deal with it, meaning that the reverse transcription would become irrelevant if the cell will be destined for certain death anyways.
If reverse transcription is a concern I think researchers need to evaluate the dynamics of protein production vs reverse transcription. Considering that the mRNA may be long lived I can see this being a concern for cells who may uptake the mRNA, not produce the spike protein, but decide to bring it back into the nucleus. But again there's plenty of other dynamics to assess there as well.
Right, it was so astonishing that spike was largely ignored, on its own and in terms of toxicity from the spike and the mRNA metabolic disruption - ER stress again. And again this is why it would have been illuminating to see results for an alternate mRNA payload...
The cmRNA vaccines are coded with Pseudouridine, an isomer of Uridine. This means that the spike proteins will be isomeric as well (as will the antibodies). The result of this is that the situation is much worse than that described in the paper: the heat shock proteins.
Sars-Cov-2 and M. bovis include identical genome sequences which include heat shock proteins. During a thermal shock, these proteins will be activated (as will the binding isomeric antibodies), with huge consequences on the DNA transcription phenomenon.
“The response to thermal stress in yeast is one of the most dynamic examples of transcriptional control known. Within 1 to 5 min of temperature upshift (30°C to 39°C), dramatic changes in protein-DNA interactions take place within HSP gene promoters, and these are accompanied by equally dramatic increases in transcription.”
“Lipid mediated gene transfer (lipofection) has been widely used to transfer genes into various cell types (1–4). Lipofection works very well in many cell lines, resulting in high transient transfection efficiencies (our observations). However, the rate of DNA integration into the genome following lipid-mediated transfection is relatively low (5) as compared to other methods, such as retroviral systems. This inefficient integration has been thought to be a major disadvantage of plasmid vectors and has limited their use in gene therapy trials.
We have attempted to overcome this hurdle by achieving higher rates of stable integrants in lipid-mediated transfections through treating the transfected cells with a mild heat shock.”
“Another factor commonly overlooked is the fact that bacteria produce reverse transcriptase via retrons, suggesting possible viral-bacterial interaction. Indeed, LPS is known to strongly impact the process:
“When we stimulated spleen cells with lipopolysaccharide (LPS), L1 mRNA levels apparently increased about 4-fold in the presence of AID and about 17-fold in its absence””
There is also this: “the possibility of telomerase HTERT, which is a reverse transcriptase enzyme, having any potential to insert the mRNA spike protein sequence into telomeric DNA.”
From the wmcresearch website:
"SARS-CoV-2 MAY OR MAY NOT WRITE ITSELF INTO YOUR DNA, BUT IT IS ALMOST CERTAINLY CUTTING/PASTING YOUR GENES, WRITING THEM BACK “WRONG" INTO YOUR DNA. LINE-1 ELEMENTS MUST BE TESTED IN ALL THOSE WITH COVID.
Retrotransposons (also called Class I transposable elements or transposons via RNA intermediates) are a type of genetic component that copy and paste themselves into different genomic locations (transposon) by converting RNA back into DNA through the process reverse transcription using an RNA transposition Retrotransposons can be further subdivided into two subclasses: those with Long-Terminal Repeats (LTR) and those without (non-LTR). LTR elements, also known as endogenous retroviruses (ERVs), comprise ~8 % of the human genome. LTR ) elements are thought to be inactive in the human lineage. That is, until SARS-CoV-2 activates them. This is why the HERV-K protein has never before been seen circulating in the body."
I found the wmcresearch site but am not sure which article corresponds to the quotes particularly for the HERV-K.
Viable TEs activate during demethylation, at which point they regulate cell differentiation during embryogenesis, or in immune and nerve cells where they seem to regulate memory. So you don't really want them active in a random stomach cell, the injections (and maybe most infections) could certainly prompt this directly or indirectly (via cell stress) and since the injections don't automatically destroy the cell, it's an implicit cancer risk. Cells should not be metabolically hijacked, in my view there will probably never be a non-carcinogenic mRNA tech.
The injections can NEVER be mRNA, but only cmRNA. Had the vaccines been coded with Uracil, ALL of the vaccinees would have found themselves in less than 24 hours in the ICUs with severe cases of Covid-19, since then the lethal abs (REGN10987 and B38) would have been activated in large quantities.
Is Sars-Cov-2 mycobacterium or a virus? If it is not a virus, then the fact that bacteria produce reverse transcriptase via retrons (especially M. tuberculosis) should worry the researchers much more than the paper published a few days ago.
wmcresearch
Category: Genetics
Subsections:
Covid-19 and Retrotransposons
The Emergence of the HERV-W ENV Protein in Covid-19
Thanks for the update to that post. The type of cells that a researcher uses should always be scrutinized for their ability to either mimic what could happen in human models or if they are representative of the process or intended goals overall. The cancer cells may have been used for their shorter replication and because LNPs may aggregate within the liver but the dynamics are very different when you add things to a petri dish rather than inject it and see where it carries and what the entire organism does with the compound.
Regardless, the most important thing is that someone is willing to examine criticisms and evaluate them accordingly. Honestly, someone may come by and say that my correction itself is wrong, although I can see why there may be plenty of confusion. The post wording wasn't very direct in what they meant.
Like I said the study is interesting but I think we will need more evidence to determine the dangers or harms of such a finding, if it is reproducible in human models.
Hi Brian. Do you want to help me with genetic analysis for an article I want to write? I can advertise your substack in that article and give y ou proper credit, of course.
https://twitter.com/ichudov/status/1497767825896878083
Sorry I was offline last night. I am happy to offer thoughts / feedback. I'm at brianmmowrey@gmail.com
I emailed you a private note
Thanks - replying now.
one must not forget that synthesis of the spike protein is also occurring at the same time.If presented to the adjacent cells or even in the medium ,several pathways may be activated ,be it via TLRs 2 or 4 or others such as CD 209/ L-SIGN.Another publication ( not yet peer reviewed) presented evidence for activation of HERV-W in some volunteer lymphocytes upon spike exposure.There could be also ER stress.
It's a bit of a chicken vs egg phenomenon. Would the cells be forced to produce spike protein in the short run, and then have the mRNA be reverse transcribed? Also, if the cells are made to express spike proteins on their outer membranes we may also assume that in animal or human models the immune system would be able to recognize the antigen and deal with it, meaning that the reverse transcription would become irrelevant if the cell will be destined for certain death anyways.
If reverse transcription is a concern I think researchers need to evaluate the dynamics of protein production vs reverse transcription. Considering that the mRNA may be long lived I can see this being a concern for cells who may uptake the mRNA, not produce the spike protein, but decide to bring it back into the nucleus. But again there's plenty of other dynamics to assess there as well.
Right, it was so astonishing that spike was largely ignored, on its own and in terms of toxicity from the spike and the mRNA metabolic disruption - ER stress again. And again this is why it would have been illuminating to see results for an alternate mRNA payload...