This series is brilliant! A bit taxing for the non-scientist (retired systems/software engineer here), but still accessible (and even entertaining) for those with an interest in figuring it out. My mind has gone through a number of theories and sources over the last 2 years. Glad I came upon your sub stack!
Essentially, "more research is required". It really is fascinating seeing you put together this information. We do have to keep in mind that the pseudouridine residues are likely to alter the longevity of the mRNA as it prevents it from being targeted by nucleases (same with the poly-A tail). I wonder if radiolabeled PEGs or mRNA may help with figuring this out.
But for now, it is "trust but verify" and "wait for further evidence".
sorry you did have a spoiler section.
This series is brilliant! A bit taxing for the non-scientist (retired systems/software engineer here), but still accessible (and even entertaining) for those with an interest in figuring it out. My mind has gone through a number of theories and sources over the last 2 years. Glad I came upon your sub stack!
Thanks for this! 👍🏽
Essentially, "more research is required". It really is fascinating seeing you put together this information. We do have to keep in mind that the pseudouridine residues are likely to alter the longevity of the mRNA as it prevents it from being targeted by nucleases (same with the poly-A tail). I wonder if radiolabeled PEGs or mRNA may help with figuring this out.
But for now, it is "trust but verify" and "wait for further evidence".